Overview
Given clarified a cell homogenate, you desire to obtain a crude preparation of nuclei, mostly free of other organelles Which one of the following techniques will allow for the efficient isolation of nuclei?
- A. Differential centrifugation
- B. Bouyant density centrifugation
- C. Rate zonal centrifugation
- D. SDS-polyacrylamide gel electrophoresis (PAGE)
- E. Immunoblotting (Western blotting)