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Biology · Studypool

Microbiology: Building A Dichotomous Key

Overview

Please help to create a Postive-Negative Table and a Flow Chart for Organism Distinguishment. To get your expected results, you will need to access Bergey’s Manual of Systematics of Archaea and Bacteria. Below is the list of the Organisms.

  • Escherichia coli
  • Shigella flexneri
  • Bifidobacterium bifidum
  • Bifidobacterium pseudocatenulatum
  • Pseudomonas aeruginosa
  • Pseudomonas fluorescens
  • Staphylococcus epidermidis
  • Staphylococcus aureus
  • Salmonella enterica
  • Bacillus megaterium
  • Proteus mirabilis
  • Enterobacter aerogenes
  • Micrococcus luteus
  • Bacillus cereus
  • Bacillus subtilis

Using the following tests:

  • Gram Stain
  • Methyl Red Test
  • Citrate Utilization
  • Motility
  • Urea Hydrolysis
  • Starch Hydrolysis
  • H2S production
  • Fermentation of different sugars (note that each sugar counts as its own test

Please see the attached files for the Details and Examples for Postive - Negative Table and Flowchart