Biology Question

Control and Sample ID Names

3 points

All control and sample ID names are included.

2 points

One sample or control or sample ID name is missing OR sample names are are not properly listed (e.g. MEH12F18-1).

1 point

More than one control or sample ID name is missing.

0 points

0 points

No control names or sample IDs are included.

Score of Control and Sample ID Names,

Expected/Predicted Results

10 points

The expected/predicted results for all PCR samples are listed correctly.

8 points

The expected/predicted results for ONE control or unknown PCR sample is not listed corrected.

6 points

The expected/predicted results for TWO control or unknown PCR samples are not listed corrected.

4 points

The expected/predicted results for MORE THAN TWO control or unknown PCR samples are not listed corrected.

0 points

No expected/predicted results are presented correctly, or the PCR slide was not included in the student's submission.

Score of Expected/Predicted Results,

Relevance of information

5 points

All tabular data are relevant. No unnecessary information is presented.

4 points

The table includes ONE additional irrelevant item (e.g. amounts of water, primers, template, etc)

3 points

The table includes TWO additional irrelevant item (e.g. amounts of water, primers, template, etc)

1 point

The table includes THREE or more additional irrelevant item (e.g. amounts of water, primers, template, etc)

0 points

There was no PCR slide included in the student's submission.

Score of Relevance of information,

Lane Labeling

10 points

All lanes are labeled appropriately.

8 points

One lane is not labeled appropriately.

6 points

Two lanes are not labeled appropriately.

4 points

Three lanes are not labeled appropriately.

2 points

Four or more lanes are not labeled appropriately.

0 points

No lanes are labeled.

Score of Lane Labeling,

Ladder Band Labeling

5 points

The bands in the DNA ladder are labeled appropriately.

4 points

The bands in the DNA ladder are labeled but at least ONE of the labels is not appropriate OR cannot be easily read (too small or crowded) OR no units are included.

2 points

The bands in the DNA ladder are labeled but at TWO-THREE of the labels is not appropriate OR cannot be easily read (too small or crowded) OR no units are included.

1 point

The bands in the DNA ladder are labeled but at FOUR of the labels are not appropriate OR cannot be easily read (too small or crowded) OR no units are included.

0 points

0 points

No labeling of the DNA ladder is presented.

Score of Ladder Band Labeling,

PCR Product Bands

16 points

Insect CO1 and Wolbachia 16S rRNA bands are labeled appropriately.

12 points

At least ONE-TWO of the following is missing OR labeled incorrectly: Insect CO1 label, 708bp, Wolbachia 16S rRNA label, 438bp.

8 points

THREE-FOUR of the following is missing OR labeled incorrectly: Insect CO1 label, 708bp, Wolbachia 16S rRNA label, 438bp.

4 points

MORE THAN FOUR of the following is missing OR labeled incorrectly: Insect CO1 label, 708bp, Wolbachia 16S rRNA label, 438bp.

0 points

0 points

No labels on the Insect CO1 or Wolbachia 16S rRNA PCR products are presented.

Score of PCR Product Bands,

Agarose and Voltage

4 points

Gel electrophoresis conditions are accurately presented.

2 points

Either the percentage of agarose in the gel OR the voltage is not presented accurately or is missing.

0 points

0 points

0 points

0 points

Neither the percentage of agarose in the gel nor the voltage is presented OR both are inaccurately presented.

Score of Agarose and Voltage,

Evaluation of Prediction

6 points

Presentation clearly and correctly describes how the results support or do not support the prediction.

4 points

Student data are not clearly linked to the prediction, and/or student did not clearly describe whether the prediction was supported or not.

2 points

Student data are not linked to the prediction, and/or student did not clearly describe whether the prediction was supported or not.

0 points

Student did not evaluate the prediction after completing the experiment.

Score of Evaluation of Prediction,

Relevance of Conclusions

6 points

Relevance of conclusions is discussed as it relates to information obtained from peer-reviewed scientific journal articles and the local collection data.

4 points

An attempt is made to discuss the relevance of the conclusions as they relate to data obtained from peer-reviewed scientific journal articles OR the local collection data.

2 points

An attempt is made to discuss the relevance of the conclusions as they relate to data obtained from peer-reviewed scientific journal articles OR the local collection data but needs significant improvement.

0 points

Relevance of the conclusions was not discussed.

Score of Relevance of Conclusions,

Reflection

3 points

A thoughtful, unique reflection is presented in detail.

2 points

A reflection is presented but is somewhat detailed, unique, or thoughtful.

1 point

A reflection is presented but is not detailed, unique, or thoughtful.

0 points

No reflection is presented.

Score of Reflection,

Slide Titles

2 points

All slides have appropriate, descriptive titles.

1 point

One slide title is missing OR One slide title is inappropriate.

0 points

Both slide titles are missing OR Both slide titles are inappropriate OR One slide title is missing and the other title is inappropriate OR No slide titles are present.

Score of Slide Titles,

Information Flow

2 points

Information in the slides is organized and clearly relays the expected or actual results.

1 point

Information in the slides is not well organized or there is additional distracting information included (e.g., unnecessary arrows, lines, or animations)

0 points

Information in the slides was poorly organized and unclear.

Score of Information Flow,

Amount of Text

2 points

Appropriate amount of text was included on the slides.

1 point

Too much or too little text was included on ONE slide.

0 points

Too much or too little text was included on BOTH slides.

Score of Amount of Text,

Font Size and Style

2 points

Font size is appropriate. Font style is consistent.

1 point

Slides show minor issues with Fonts: Font size is too large, too small, or inconsistent.

0 points

Font size is too large, too small, or inconsistent in multiple areas of slides.

Score of Font Size and Style,

Spelling and Grammar

2 points

No spelling or grammar errors exist.

1 point

Some spelling or grammar problems exist.

0 points

Many problems with spelling and/or grammar exist.

Score of Spelling and Grammar,

Image Appearance (Expected Results)

2 points

Expected results are presented in tabular format AND Table is created using PowerPoint software.

1 point

Results are presented but are not in tabular format OR Screenshot of table from the lab manual is inserted.

0 points

No expected results are presented.

Score of Image Appearance (Expected Results),

Image Appearance (Gel Results)

2 points

Gel Image is a good size, appropriately cropped, focused and not distorted.

1 point

The gel image had a problem with ONE of the following: not the appropriate size, not cropped appropriately, not clear (was distorted).

0 points

The gel image had a problem with MORE THAN ONE of the following: not the appropriate size, not cropped appropriately, not clear (was distorted) OR Gel image was not included.

Score of Image Appearance (Gel Results),

Scientific Names

2 points

Scientific names are written correctly.

1 point

Scientific names are written incorrectly.

0 points

No scientific names are included.

Score of Scientific Names,