CRISPR-Cas techniques in plants or animals or humans

Post ID: 3374505

Overview

With the advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR- associated protein (Cas) mediated genome editing, the molecular and functional mechanism of the gene has progressed significantly in recent years. In this genome editing tool, CRISPR-associated Cas nucleases are restricted to their target of DNA by their preferred protospacer adjacent motifs (PAMs). A number of CRISPR-Cas variants have been developed e.g. CRISPR-Cas9, -Cas12a, and -Cas12b, with different PAM requirements. CRISPR technology is widely applied in different areas such as plant improvement and knock-out DNA in research mice and rats, silenced and the defective gene for human disease is knocked in. The writing assignment discusses the main application of CRISPR-Cas and the significance of such an application useful in the research area.

Following the guidelines for writing the mini-review assignment.

A. Each student should select FIVE scientific papers research/review on the specific topics of CRISPR-Cas techniques in plants or animals or humans.

B. You will find FIVE papers as an examples which may help you. (Bhowmik et al., 2021; Cardi et al., 2023; Deng et al., 2022; Galli et al., 2022; Geisslitz et al., 2022; Khan et al., 2022; Naik et al., 2022; Riaz et al., 2022; Zaidi et al., 2020)

C. Then use the selected papers to write EIGHT to TEN -word pages (without cover page and references) with figures and tables it can be up to TWELEV pages (maximum 1 table and 1 figure).

D. Should use Times New Roman (font type) and 12 (font size) with 1.5 space.

E. The writing assignment should provide a concise summary of a specific research topic or field relevant to CRISPR-Cas techniques. Explain the previous research and findings in context and present current developments in a critical and focused manner.

F. The topic of the application of CRISPR-Cas techniques is preferred.

G. The mini-review should be written based on the provided formatting and scientifically, i.e.

Title, Abstract (150 words), keywords (up to six words)

Introduction (including objectives)

Discussion or text matter (headings, subheadings)

Conclusions plus references

Preparing your writing assignment

Title page

Abstract

Keywords

Background (Introduction)

Main text (Discussion)

Conclusions with future perspective

Figure, and table

References, please use APA 7th style

These are examples of papers

Riaz, A., Kanwal, F., Ahmad, I., Ahmad, S., Farooq, A., Madsen, C. K., Brinch-Pedersen, H., Bekalu, Z. E., Dai, F., Zhang, G., & Alqudah, A. M. (2022). New Hope for Genome Editing in Cultivated Grasses: CRISPR Variants and Application. Front Genet, 13, 866121. https://doi.org/10.3389/fgene.2022.866121

Bhowmik, P., Konkin, D., Polowick, P., Hodgins, C. L., Subedi, M., Xiang, D., Yu, B., Patterson, N., Rajagopalan, N., Babic, V., Ro, D.-K., Tar'an, B., Bandara, M., Smyth, S. J., Cui, Y., & Kagale, S. (2021). CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges. Legume Science, 3(3), e96. https://doi.org/https://doi.org/10.1002/leg3.96

Cardi, T., Murovec, J., Bakhsh, A., Boniecka, J., Bruegmann, T., Bull, S. E., Eeckhaut, T., Fladung, M., Galovic, V., Linkiewicz, A., Lukan, T., Mafra, I., Michalski, K., Kavas, M., Nicolia, A., Nowakowska, J., Sagi, L., Sarmiento, C., Yildirim, K., . . . Van Laere, K. (2023). CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation. Trends Plant Sci, 28(10), 1144-1165. https://doi.org/10.1016/j.tplants.2023.05.012

Deng, F., Zeng, F., Shen, Q., Abbas, A., Cheng, J., Jiang, W., Chen, G., Shah, A. N., Holford, P., Tanveer, M., Zhang, D., & Chen, Z. H. (2022). Molecular evolution and functional modification of plant miRNAs with CRISPR. Trends Plant Sci, 27(9), 890-907. https://doi.org/10.1016/j.tplants.2022.01.009

Galli, M., Martiny, E., Imani, J., Kumar, N., Koch, A., Steinbrenner, J., & Kogel, K. H. (2022). CRISPR/SpCas9- mediated double knockout of barley Microrchidia MORC1 and MORC6a reveals their strong

references and without cover page) by 30 April, 2025, at 11:59 pm.

involvement in plant immunity, transcriptional gene silencing and plant growth. Plant Biotechnol

J, 20(1), 89-102. https://doi.org/10.1111/pbi.13697

Geisslitz, S., Islam, S., Buck, L., Grunwald-Gruber, C., Sestili, F., Camerlengo, F., Masci, S., & D’Amico, S.

(2022). Absolute and relative quantitation of amylase/trypsin-inhibitors by LC-MS/MS from wheat lines obtained by CRISPR-Cas9 and RNAi. Frontiers in Plant Science, 13, 974881. https://pmc.ncbi.nlm.nih.gov/articles/PMC9465248/p...

Khan, S. H., Tariq, H., Farooq, I., Tasleeem, H., Ghouri, M. Z., Mubarik, M. S., & Khan, Z. (2022). Applications of CRISPR/Cas System in Plants. In A. Ahmad, S. H. Khan, & Z. Khan (Eds.), The CRISPR/Cas Tool Kit for Genome Editing (pp. 285-309). Springer Singapore. https://doi.org/10.1007/978-981-16-6305- 5_9

Naik, B. J., Shimoga, G., Kim, S.-C., Manjulatha, M., Subramanyam Reddy, C., Palem, R. R., Kumar, M., Kim, S.-Y., & Lee, S.-H. (2022). CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement [Review]. 13. https://doi.org/10.3389/fpls.2022.843575

Zaidi, S. S., Mahas, A., Vanderschuren, H., & Mahfouz, M. M. (2020). Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants. Genome Biol, 21(1), 289. https://doi.org/10.1186/s13059-020-02204-y