Answer The Following Questions

Overview

Transfection Efficiency Calculation

In this assignment, HeLa cells are transfected with different concentrations of plasmid expressing GFP. You will calculate the efficiency of transfection and determine how transfection efficiency changes with increase in plasmid concentration.

Please show calculations and work shown where applicable to receive full credit. This practice is essential for verifying your understanding of the material and ensuring fairness for all students. This also allows us to award you partial credit by demonstrating you understand the methodology, even though your numerical values might not be correct. Failure to provide complete work will result in point deductions.

Cell culture assignment

This assignment allows students to understand the concept of passaging or splitting cells. It teaches you how to seed cells into dishes, in order to prepare confluent cell cultures for experiments.

Please show calculations and work shown where applicable to receive full credit. This practice is essential for verifying your understanding of the material and ensuring fairness for all students. This also allows us to award you partial credit by demonstrating you understand the methodology, even though your numerical values might not be correct. Failure to provide complete work will result in point deductions.

Cell Viability: Trypan Blue Exclusion

Please show calculations and work shown where applicable to receive full credit. This practice is essential for verifying your understanding of the material and ensuring fairness for all students. This also allows us to award you partial credit by demonstrating you understand the methodology, even though your numerical values might not be correct. Failure to provide complete work will result in point deductions.